Instruction No. 4.2.2123-06 of the Chief Sanitary Inspector regarding the control of cattle blood serum for extraneous viruses and mycoplasms. Country/Territory Russian Federation Document type Regulation Date 2006 Source FAO, FAOLEX Subject Livestock Keyword Cattle Biotechnology Geographical area Arctic, Asia and the Pacific, Black Sea, Caspian Sea, CIS (Commonwealth of Independent States), Eastern Europe, Europe, Europe and Central Asia, North Pacific, North-West Pacific Abstract This Instruction establishes the methods by which cattle blood serum shall be controlled for the presence of extraneous viruses and mycoplasms. Cattle blood serum is widely used for the manufacturing of prophylactic and diagnostic immunobiological preparations and for the cultivation of cell cultures. Cattle blood serum control consists of two methods: (a) microbiological method based upon a diagnosis of mycoplasm growth after the introduction of serum under test into growth medium with a diagnosis period of 21 days; and (b) indicator cell culture method (cytochemical method) based upon diagnosis of DNA mycoplasms coloured by fluorochrome Hoechst-33258 on indicator cell culture Vero with a diagnosis period of three to five days. Full text Russian Website www.lawrussia.ru